Confirmatory Method for Determination of Coccidiostats in Eggs
نویسندگان
چکیده
The method for confirmatory analysis of clazuril, decoquinate, diclazuril, halofuginone, lasalocid, maduramicin, monensin, narasin, nicarbazin, robenidine, salinomycin, and semduramicin in eggs has been developed and validated. The samples were extracted with acetonitrile, purified on Oasis HLB SPE columns and analysed by liquid chromatography coupled with tandem mass spectrometry. The validation, performed according to the EU requirements, proved that the method enables the confirmation of coccidiostats in eggs at the levels required in the official control of residues (CCα in the range of 2.92-178 μg/kg, depending on the coccidiostat). The within-laboratory reproducibility (CV in the range of 6.1-29.3%) is also acceptable. The method was successfully verified in the proficiency test and implemented in the national residue control plan.
منابع مشابه
Development of a five-plex flow cytometric immunoassay for the simultaneous detection of six coccidiostats in feed and eggs
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